在人类子宫内膜 stromal 细胞 decidualization 期间的 lysosome 动态:对 para-nonylphenol 的作用
Sofia Passaponti1,2, Camilla Manzan Martins1, Laura Cresti1
1Department of Life Sciences, University of Siena, Siena, Italy.
American journal of physiology. Cell physiology
|May 13, 2024
概括
帕拉-诺尼尔 (p-NP) 是一种内分泌干扰物,它会破坏人类子宫内膜层细胞的分离. 这会影响 lysosome 功能和细胞外矩阵重塑,可能阻碍胚胎植入和怀孕成功.
科学领域:
- 生殖生物学和毒理学.
- 内分泌学和细胞信号传递
背景情况:
- 脱二化对于胚胎植入至关重要,涉及到动态的子宫内膜 stromal 细胞变化.
- 溶酶体活动与怀孕早期子宫内膜的生理变化有关.
- 内分泌干扰剂可能会干扰生殖过程.
研究的目的:
- 在决定化过程中,研究帕纳尼尔 (p-NP) 对人类永生性子宫内膜层细胞 (tHESCs) 的作用.
- 评估p-NP对决定化标记物,溶酶体生物发生和外细胞形成以及细胞外矩阵重塑的影响.
主要方法:
- 对p-NP (1 nM和1 pM) 的暴露.
- 对决定化标记物的分析 (连-43,IGFBP1,益生菌).
- 研究溶酶体生物发生/外细胞分裂 (TFEB,LAMP-1,cathepsin D活性).
- 评估细胞外矩阵重塑 (纤维蛋白试验).
主要成果:
- p-NP降低了益生素表达和受损的决定性标志物.
- p-NP增加了TFEB的核局部化和LAMP-1的转位,表明增强了溶酶体生物发生和外细胞形成.
- 增加的甲素D释放和改变的纤维内素表明细胞外基质重塑.
结论:
- 帕拉尼尔会对人类子宫内膜层细胞的脱产生负面影响.
- p-NP 破坏了 lysosome 功能和细胞外矩阵重塑,可能危及胚胎植入.
- 这些发现凸显了内分泌干扰剂对生殖成功的不利影响.
相关概念视频
01:31Lysosomes
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
01:22Lysosomal Hydrolases
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...