Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Conserved Binding Sites01:49

Conserved Binding Sites

4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

10.8K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.8K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Protein Families02:47

Protein Families

15.3K
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism.   Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members.   If these new proteins contain similar amino acids in key...
15.3K
Conservation of Protein Domains02:26

Conservation of Protein Domains

3.1K
3.1K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

SENP1 drives glycolytic reprogramming to promote malignant progression of gastric cancer via deSUMOylation of HIF-1α.

Tissue & cell·2026
Same author

Safety and efficacy of cytokine-induced killer cells for gastric cancer: a systematic review and meta-analysis.

Frontiers in oncology·2026
Same author

EC-Dock: A Fast Equivariant Consistency Model for Molecular Docking and Virtual Screening.

Journal of chemical information and modeling·2026
Same author

Mechanism of action of Houttuynia cordata extract and its application in animal production.

Frontiers in veterinary science·2026
Same author

Photoinduced H-Bonding EDA complex-enabled skeletal editing of furans to pyridazines.

Nature communications·2026
Same author

Developing an integrated dementia care model using service blueprinting: process visualization and failure point analysis.

BMC health services research·2026
Same journal

Cap 2'-O-methyltransferase CMTR2 regulates male meiosis independent of its methyltransferase activity.

Nucleic acids research·2026
Same journal

APE1 binds and processes abasic sites present in i-motif DNA and cooperates with PCBP1 in maintenance of telomeric stability.

Nucleic acids research·2026
Same journal

Acquisition of a novel restriction modification system regulates genetic flux and gene expression in the hypervirulent and globally disseminated CC17 lineage of group B Streptococcus.

Nucleic acids research·2026
Same journal

Trans-species microRNAs from the parasitic plant Cuscuta campestris specifically avoid loading onto self Argonautes.

Nucleic acids research·2026
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
查看所有相关文章

相关实验视频

Updated: Jun 26, 2025

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

68.7K

GPSFun:用语言模型进行几何意识的蛋白质序列功能预测.

Qianmu Yuan1, Chong Tian1, Yidong Song1

  • 1School of Computer Science and Engineering, Sun Yat-sen University, Guangzhou, Guangdong 510000, China.

Nucleic acids research
|May 13, 2024
PubMed
概括
此摘要是机器生成的。

GPSFun是一个新的Web服务器,它使用语言模型和几何深度学习来进行蛋白质功能注释. 它只从序列中准确预测蛋白质功能,推动药物发现和疾病机制研究.

更多相关视频

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.3K

相关实验视频

Last Updated: Jun 26, 2025

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

68.7K
Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.3K

科学领域:

  • 计算生物学是一种计算生物学.
  • 生物信息学是一种生物信息学.
  • 结构生物学是结构生物学.

背景情况:

  • 了解蛋白质功能对于疾病研究和药物向鉴定至关重要.
  • 在蛋白质序列数据的快速增长和功能注释的可用性之间存在很大的差距.
  • 之前的方法如GraphPPIS,GraphSite,LMetalSite和SPROF-GO已被开发用于蛋白质功能预测.

研究的目的:

  • 为了介绍GPSFun,一个多功能Web服务器,用于几何意识的蛋白质序列函数注释.
  • 整合语言模型和几何深度学习以增强蛋白质功能预测.
  • 为各种下游功能预测提供一个用户友好的平台.

主要方法:

  • 使用大型语言模型来预测3D蛋白质构造和提取序列嵌入.
  • 使用几何图形神经网络来分析蛋白质图中的序列和结构模式.
  • 开发一个具有直观接口和可访问性可视化的Web服务器.

主要成果:

  • 与最先进的方法相比,GPSFun在各种预测任务上表现出卓越的性能.
  • 服务器准确地预测了蛋白质连接体结合点,基因本体学,亚细胞位置和蛋白质溶解度.
  • 有效的函数注释可以实现,而不依赖于多个序列对齐或实验结构.

结论:

  • GPSFun提供了一种强大而通用的工具,用于使用基于序列的预测进行蛋白质功能注释.
  • 集成先进的人工智能技术解决了在大型蛋白质序列数据集中功能注释有限的挑战.
  • GPSFun是免费访问的,支持分子生物学,药物发现和疾病机制阐明的更广泛的研究.