SAR-endolysin LysKpV475与polymyxin B和沙门氏菌菌体phSE-5的联合作用
Marco Gontijo1,2, Mateus Pereira Teles1,3,4, Hugo Martins Correia1
1Departamento de Genética, Evolução, Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP 13083-862, Brazil.
Microbiology (Reading, England)
|May 13, 2024
概括
这项研究探讨了SAR-endolysin LysKpV47575的SAR-endolysin.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 菌体 (菌体) 编码的内素会降解细菌的酸盐.
- 再组合内氨酸对克拉姆阳性细菌有效.
- 格拉姆阴性细菌的外膜阻碍了内溶素的有效性.
研究的目的:
- 评估SAR-endolysin LysKpV475对格兰阴性细菌的抗菌活性.
- 评估LysKpV475作为单一或联合治疗.
- 为了研究LysKpV475与多素B或菌体结合,自由或固定.
主要方法:
- 单独测试了LysKpV475和polymyxin B对十种格拉姆阴性细菌进行了测试 (体外和体内).
- 结合溶解的LysKpV475与菌体phSE-5 (自由或在pullulan矩阵) 对抗沙门氏菌Typhimurium.
- 确定了细菌静止和杀菌活性,以及分数抑制度指数 (FICI).
主要成果:
- LysKpV475对P. aeruginosa,S. enterica Typhimurium,K. pneumoniae和E. cloacae进行了细菌静止活动.
- 对P. aeruginosa菌株观察到杀菌活性.
- 与聚米辛B的组合增强了对K. pneumoniae和S. enterica Typhimurium的活性 (FICI <1.0).
- 观察到与菌体phSE-5对抗S. enterica Typhimurium的协同效应.
- 在Pullulan矩阵中的LysKpV475在6小时后将沙门氏菌减少了2个日志.
结论:
- SAR-endolysins显示出对抗格拉姆阴性细菌的潜力.
- 与多素B或菌体的组合可以提高疗效.
- 固定化的内素保留了抗微生物活性.
- 在生物控制,生物卫生和食品保存方面的潜在应用.
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