相关实验视频
Updated: Jun 26, 2026

10:33
Generation of Alginate Microspheres for Biomedical Applications
Published on: August 12, 2012
20.8K
聚乙胺含量用于开发基于酸的水性核心外囊,用于生物医学应用
Asma Sadat Vaziri1,2, Maryam Alizadeh2, Ebrahim Vasheghani-Farahani1
1Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran 14115-111, Iran.
ACS applied materials & interfaces
|May 13, 2024
概括
这项研究引入了基于酸盐的新型液体核心外囊,用于3D细胞培养. 这些改进的囊增强结构稳定性,促进生物医学应用的细胞生长.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 药物运输 药物运输 药物运输
背景情况:
- 水性核心-外结构促进了3D球形的形成,与生物体内类似的细胞与细胞接触.
- 酸盐 (ALG) 囊在结构稳定性和细胞识别方面存在局限性.
- 聚乙烯胺 (PEI) 具有增强酸盐基系统的潜力.
研究的目的:
- 开发一种新的策略,利用酸盐,聚乙烯胺和酸盐二甲制造稳定的液体核心囊.
- 研究制造囊的结构,机械和生物特性.
- 评估这些囊作为3D细胞培养平台的潜力.
主要方法:
- 囊的制造方法是通过对酸盐进行逆凝,并用聚乙烯胺和酸盐二甲基化物逐层涂层.
- 使用扫描电子显微镜和光显微镜进行表征.
- 评估机械性能 (有效模量),抗菌活性,细胞活力和球形形成.
主要成果:
- 成功制造了外径增加的球形空心囊 (ALG/PEI/ADA).
- 与裸体ALG囊相比,PEI和PEI/ADA修饰囊的有效模量显著增加.
- PEI涂层囊表现出抗菌特性,所有囊都显示出细胞相容性.
- 封装细胞繁殖并形成球形,在5天内厚度增加.
结论:
- 开发的酸盐/PEI/ADA囊提供增强的结构稳定性和细胞兼容性.
- 这种新的封装协议为3D细胞培养提供了一个有效的体外平台.
- 这种可适应的系统对各种生物医学应用具有前景,包括组织工程和药物输送.
相关概念视频
Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Synthesis of Phosphatidylcholine in the ER Membrane
The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...
The major components of all eukaryotic cell...
Carboxylic Acid Derivatives: Overview
Carboxylic acid derivatives are formed by replacing the hydroxyl group of carboxylic acids with a different functional group. The most common carboxylic acid derivatives are:
Extracellular Matrix
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Preclinical Development: Overview
Preclinical development consists of a series of tests that ensure the safety and efficacy of a new therapeutic compound before it is tested in humans. There are four main phases to this process. First, safety pharmacology tests are conducted to ensure the drug does not produce any acutely harmful effects. These tests examine parameters such as bronchoconstriction, cardiac dysrhythmias, blood pressure changes, and ataxia. Next, preliminary toxicological testing is performed to determine the...
Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

