外源黄减轻了菜植物中的压力:生物化学和代谢学调查
Leilei Zhang1, Gokhan Zengin2, Ceyda Ozfidan-Konakci3
1Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, 29122, Piacenza, Italy.
Plant physiology and biochemistry : PPB
|May 13, 2024
概括
黄素通过改善光合作用性能和水关系,有效地减轻了菜中的毒性. 它还增强了抗氧化能力,调节了植物代谢,减少了氧化应激.
科学领域:
- 植物生理学 植物生理学
- 环境毒理学环境毒理学
- 生物化学 生物化学
背景情况:
- (As) 污染对作物生产率和食品安全构成重大威胁.
- 了解植物对压力的反应,对于制定减缓策略至关重要.
- 黄素 (Cur) 是一种天然化合物,已证明具有抗氧化和保护性质.
研究的目的:
- 调查外源性黄素对菜 (S. oleracea L.) 中素诱导的毒性减轻作用.
- 探索库尔库明对抗压力的保护作用背后的生理生化机制.
- 为了确定关键的代谢途径和生物标志物调节的素在压力菜.
主要方法:
- 在与接触的同时,对菜植物进行了不同度的黄素 (1,10,20μM) 的处理.
- 测量了光合作用性能,相对含水量,透气率,口腔导电性和碳同化.
- 评估了氧化应激生物标志物 (H2O2,MDA) 和抗氧化能力.
- 使用非向代谢物,AMOPLS-DA和rCCA来分析代谢变化和相关性.
主要成果:
- 黄素显著改善了在氧压力下菜的光合作用性能.
- 库尔库恢复了叶子的相对水含量和透潜力,并增强了透气,口腔导电性和碳同化.
- 黄素减少了氧化应激生物标志物,并增加了非酶体抗氧化能力.
- 代谢分析揭示了黄素诱导的葡萄糖酸盐,化合物和增强的谷氨氧化还原循环活性的积累.
- 相关性分析表明,特定代谢物 (例如 (+) - 二甲,L-氨酸) 和抗氧化活性之间存在联系,这表明ROS信号与植物激素之间的交叉对话.
结论:
- 外源性黄素有效地减轻了在菜中引起的生理化学压力.
- 黄素增强了植物的水状态,光合作用效率和抗氧化剂防御机制.
- 黄调节植物的二次新陈代谢,促进保护性化合物的积累和激活抗氧化途径.
- 黄素的保护作用涉及到反应性氧物种信号传递,植物激素和代谢调整之间的复杂相互作用.
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