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兰普雷VDAC2:通过降低BAK表达的调节,抑制过氧化诱导的293T细胞亡

Mingjian Zhang¹, Wenwei Li¹, Xue Zhang¹

¹College of Life Sciences, Lamprey Research Center, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University, Dalian, 116081, China.

Fish & shellfish immunology

|May 13, 2024

概括

兰普雷电压依赖性离子通道2 (Lr-VDAC2) 存在于线粒体中,并抑制了亡. 这一发现揭示了脊椎动物中VDAC2保留的抗亡作用,影响细胞生存机制.

科学领域:

线粒体生物学线粒体生物学
细胞信号传输细胞信号传输
进化生物学进化生物学

背景情况:

电压依赖性离子通道2 (VDAC2) 是一个关键的线粒体外膜蛋白,调节运输和细胞过程,如细胞亡.
了解不同物种中的VDAC2功能,可以了解保存的生物机制.
下脊椎动物为蛋白质功能的进化保护提供了独特的视角.

研究的目的:

为了识别和表征下脊椎动物的VDAC2同类, (Lethenteron reissneri).
为了研究Lamprey VDAC2 (Lr-VDAC2) 在细胞过程中的局部化,表达模式和功能作用.
为了阐明LR-VDAC2.2.的抗亡机制.

主要方法:

鉴定和测序分析Lr-VDAC2从lamprey.
遗传学分析以确定进化关系.
使用灯细胞进行亚细胞局部化研究.
在不同的灯组织中对Lr-VDAC2表达的定量分析.
使用293T细胞进行功能测试,以评估细胞亡抑制和西部抹杀,以分析关键的细胞亡蛋白 (Caspase 3,Caspase 9,细胞 C,BAK).

主要成果:

Lr-VDAC2,一个具有>50%序列相同性的VDAC2同类物,在鱼中被确定并局部化到线粒体中.
Lr-VDAC2表达在诸如,肌肉和心肌等能源密集型组织中最高.
通过降低caspase3,caspase9和cytochrome C的调节,lr-VDAC2显著抑制过氧化诱导的亡.
在翻译层面上,lr-VDAC2抑制了亲亡蛋白BAK,显示出一种抗亡功能.

结论:

Lr-VDAC2是人类VDAC2的功能同类物,在下脊椎动物中保存.
这项研究揭示了VDAC2的保守抗亡作用,这种作用是由抑制BAK活性介导的.
这些发现凸显了VDAC2在不同脊椎动物物种中调节细胞存活的进化重要性.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.这里是巴克巴克巴克巴克.兰普雷兰普雷是什么意思在VDAC2中,它是VDAC2.

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