免疫吸附作为在COVID-19大流行期间抗体捐赠的方法
Jannik Rothenburg1,2, Silke Rink-Baron3, Lisa Müller4
1Institute for Transplant Diagnostics and Cell Therapeutics, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany.
Vox sanguinis
|May 13, 2024
概括
免疫吸附和触流过有效地将SARS-CoV-2抗体从康复期血中缩起来. 这种方法产生适合潜在的针对SARS-CoV-2的被动免疫策略的高滴度抗体缩物.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 病毒学 病毒学
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 感染需要有效的治疗干预措施.
- 康复性血疗法,含有抗SARS-CoV-2抗体,是早期治疗策略.
- 高中和抗体标位对于康复期血治疗的有效性至关重要.
研究的目的:
- 为了评估免疫吸附 (IA) 与接触流过 (TFF) 结合用于抗体度生产.
- 证明IA和TFF产生具有高中和能力的抗体度的能力.
- 评估获得潜在治疗用途的缩抗体产品的可行性.
主要方法:
- 10名捐赠者使用特定抗体吸附柱进行了免疫吸附.
- 使用30kDa膜的触流流过方法,化了抗体的度.
- 通过透过的方式,将甘氨酸缓冲剂换成正常盐水.
主要成果:
- 抗体度在8小时内产生,显示总IgG,IgA和IgM水平显著升高.
- 缩物显示出SARS-CoV-2 N和S抗体的度大幅增加,包括S特异性IgG.
- 在大多数缩物中,特定的SARS-CoV-2病毒中和能力增加,这些缩物在微生物学上安全且稳定一年.
结论:
- 免疫吸附接着触流过是生产低容量抗体度的可行方法.
- 这些缩物含有丰富的IgG,IgA,IgM和SARS-CoV-2特异性抗体,没有白蛋白和凝固因子.
- 需要进一步调查,以确定这些抗体度在感染SARS-CoV-2患者的被动免疫中具有治疗功效.
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