氧化光催化在膜上触发非正规的热灭菌
Chaiheon Lee1,2,3, Mingyu Park1,2, W C Bhashini Wijesinghe1
1Department of Chemistry, School of Natural Science, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea.
Nature communications
|May 13, 2024
概括
氧化应激向细胞内膜蛋白,引发一种新型的细胞死亡形式,称为热亡. 这项研究揭示了破坏这些蛋白质如何启动导致细胞溶解的级联过程.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 生物物理学的生物物理.
背景情况:
- 细胞内膜蛋白对于真核细胞的功能至关重要.
- 氧化应激对细胞内膜的影响尚不清楚.
研究的目的:
- 为了研究细胞对氧化应激的反应,专注于细胞内膜蛋白.
- 探索将膜蛋白氧化与细胞死亡联系起来的机制.
主要方法:
- 针对有针对性的氧化损伤的两性光催化剂的开发.
- 使用单分子磁子来评估蛋白质折叠的稳定性.
- 使用无标签量化来识别细胞环境中受损的蛋白质.
主要成果:
- 光催化诱导了细胞内膜蛋白的氧化损伤,破坏了它们的折叠.
- 氧化损伤主要影响参与质量控制的蛋白质,增加线粒体和内质网膜中的压力.
- 这一过程激活了非正规的炎症酶,导致气体皮质D裂变和热亡.
结论:
- 细胞内膜蛋白的氧化是非正规性热的触发因素.
- 对膜的有针对性的氧化应激可以通过新的途径诱导溶性细胞死亡.
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