工程非传统酵母Rhodotorula toruloides用于ergothioneine生产的工程酵母
Ke Liu1, Gedan Xiang1, Lekai Li1
1State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Biotechnology for biofuels and bioproducts
|May 14, 2024
概括
研究人员对Rhodotorula toruloides进行了改造,以提高厄戈氨酸 (EGT) 的产生. 最终的菌株获得了显著的EGT标位,显示了工业应用的潜力.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 生物化学生产 生化生产
背景情况:
- 欧戈氨酸 (EGT) 是一种有价值的抗氧化剂,具有广泛的应用.
- 微生物发酵提供了一种可持续且具有成本效益的EGT生产方法.
- 在微生物宿主中提高EGT生物合成效率仍然是一个挑战.
研究的目的:
- 为了增强Rhodotorula toruloides中的厄戈氨酸 (EGT) 生产.
- 为了建立有效的基因组编辑和选方法R. toruloides.
- 开发一种具有工业相关EGT产量的工程菌株.
主要方法:
- 对野生型R. toruloides菌株进行EGT积累的查.
- 开发CRISPR辅助的CRE重组 (CACR) 进行代基因组编辑.
- 整合EGT生物合成基因和S-adenosylmethionine再平衡.
- 使用ergothionase进行突变选择的高通量选.
主要成果:
- Rhodotorula toruloides 2.1389 (RT1389) 显示了最高的本地EGT产量 (79.0毫克/升).
- 一种带有额外EGT基因的工程菌株 (RT1389-2) 增加了EGT标位的1.5倍.
- 最后一个工程菌株 (RT1389-3) 通过基因修饰和S-adenosylmethionine再平衡,实现了267.4 mg/L的EGT.
结论:
- CACR和高通量选使R. toruloides的快速工程能够改善EGT的生产.
- 改造的RT1389-3菌株显示出相当大的EGT生产能力.
- 这项研究为潜在的工业EGT生物生产提供了有希望的突破.
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