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纳米塑料的尺寸和表面化学决定了人类唾液蛋白的装饰

Anna Dorsch¹, Fritz Förschner¹, Mehdi Ravandeh¹

¹ZIK plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.

ACS applied materials & interfaces

|May 14, 2024

概括

人的唾液蛋白与微和纳米塑料颗粒 (MNPPs) 结合,形成一个蛋白质冠状. 粒子大小和电荷影响这种结合,影响MNPP与人体细胞的相互作用和潜在的毒性.

科学领域:

环境科学环境科学
生物化学生物化学
毒理学毒理学毒理学

背景情况:

塑料聚合物对环境的污染会产生微型和纳米塑料颗粒 (MNPP).
MNPPs进入人类的食物链,最初的接触发生在口腔中通过唾液.
在唾液中的MNPPs上形成的蛋白质冠状和其对粒子特性的依赖性基本上是未知的.

研究的目的:

为了研究蛋白质与不同大小和电荷的聚烯MNPP的结合.
确定粒子生物物理性质如何影响蛋白质冠状形成.
评估蛋白冠对MNPP与人类细胞相互作用的影响.

主要方法:

用人类唾液化聚烯MNPPs (两种尺寸,三种充电量).
动态光散射用于粒子大小和泽塔电位分析.
纳米液体染色学-高分辨率质谱学 (nLC/HRMS) 用于蛋白质冠状体的识别和量化.
用5个人类细胞系进行细胞培养研究,以评估细胞效应.

主要成果:

蛋白质冠状元的组成和数量受到MNPP大小和电荷的显著影响.
粒子特性如水力动力学大小,多分散性和泽塔潜力受到蛋白质结合的影响.
较小的MNPP表现出与唾液蛋白更大的反应性.
蛋白冠状形成调节了MNPPs对细胞代谢活动和基因毒性的影响.

结论:

MNPP的大小和表面电荷是唾液蛋白冠状形成的关键决定因素.
冠状蛋白影响MNPP与人体细胞的相互作用,影响细胞功能.
这些发现对理解MNPP吸收和人类生物效应具有重大意义.

关键词:

在HPLC中使用HPLC.遗传毒性遗传毒性遗传毒性遗传毒性质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量微塑料微塑料的使用聚乙烯是一种聚乙烯.蛋白质冠状病毒冠状病毒蛋白质组学蛋白质组学毒性的毒性毒性的毒性

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