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先进的UPLC-MS/MS方法用于SIPI6398在老鼠血中的量化及其药理动力学特性

Fan Chen¹, Shunjun Ma², Runrun Wang²

¹Ruian People's Hospital, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Journal of analytical methods in chemistry

|May 14, 2024

概括

一种新方法量化了SIPI6398在老鼠血中的含量,揭示了其药理动力学和13.2%的生物可用性. 这项研究提高了对这种新型抗精神分裂症药物的理解.

科学领域:

药理学药理学是指药理学的学科.
分析化学分析化学
神经科学是一个神经科学.

背景情况:

SIPI6398是一种新型抗精神分裂症药物,其选择性和安全性比现有治疗方法更好.
对于SIPI6398.8,存在有限的体内药理动力学和生物可用性数据.

研究的目的:

开发和验证一种快速超高性能液体色谱-并联质谱法 (UPLC-MS/MS) 方法,用于量化大鼠血中的SIPI6398.
研究SIPI6398的药理动力学并确定SIPI6398在老鼠中的生物可用性.

主要方法:

开发了一种UPLC-MS/MS方法,使用HSS T3柱与酸-水移动相和梯度化.
血样本是通过用乙二甲醇沉蛋白质来准备的.
SIPI6398被口服 (4毫克/千克) 和静脉注射 (2毫克/千克) 给大鼠进行药理学分析.

主要成果:

在1-2000 ng/mL范围内,UPLC-MS/MS方法表现出极好的线性 (r > 0.99).
该方法满足了精度,准确性,回收,矩阵效应和稳定性的所有标准.
确定SIPI6398的口服生物利用率为13.2%.

结论:

一种经过验证的UPLC-MS/MS方法可以在大鼠血中准确量化SIPI6398.
该研究为SIPI6398提供了关键的药理动力学和生物可用性数据,有助于其进一步发展为抗精神分裂症药物.

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