使用超临界流体染色学-质谱学和不同的质量分析仪来确定聚乙烯甘醇的分散性
Sergio Cancho-Gonzalez1, Paul Ferguson2, Julie M Herniman1
1Faculty of Engineering and Physical Sciences, School of Chemistry, University of Southampton, Southampton, UK.
Rapid communications in mass spectrometry : RCM
|May 14, 2024
概括
使用低分辨率仪器对聚乙烯糖醇配方的质谱分析提供了准确的分散值. 整合一个单分散的内部标准可以提高方法的精度,而不会影响结果.
科学领域:
- 聚合物化学 聚合物化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 分散值是聚乙烯糖醇 (PEG) 配方的关键质量属性,影响药物的性能.
- 质谱法 (MS) 对PEG的分析可能会受到质量分析仪设计的影响,影响分散度的测量.
- 需要进一步了解MS分析仪对PEG分散性的影响.
研究的目的:
- 为了比较使用不同质量分析仪的聚乙烯糖醇的分散值计算.
- 评估PEG寡合体的响应选择和定量校准方法.
- 评估内部标准对PEG分散度测量的扩大不确定性的影响.
主要方法:
- 与各种质量分析仪连接的超临界流体染色学 (SFC) 用于分析PEG.
- 分析了解密的电子喷雾电离质谱.
- 评估了五种校准方法和使用单分散内部标准 (ISTD).
主要成果:
- 低分辨率的质量分析仪提供了与高分辨率PEG分散仪器相似的量化能力.
- 使用替代寡合体或整个分布的校准方法产生了一致的结果,表明恒定的电离效率.
- 超临界流体色谱的高分辨率使得使用单分散的ISTD可以提高准确性和精度.
结论:
- 低分辨率的质量分析仪足以准确和精确地确定PEG分散度.
- 由于离子质量分辨率优越,建议使用高分辨率仪器进行详细的表征.
- 一个单分散的ISTD提高了精度,没有分析物抑制,保持分散值的准确性.
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