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相关概念视频

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
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相关实验视频

Updated: Jun 26, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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通过SID1识别双链RNA的结构基础.

Runhao Wang1, Ye Cong2, Dandan Qian1

  • 1State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin 300350, China.

Nucleic acids research
|May 14, 2024
PubMed
概括
此摘要是机器生成的。

研究人员阐明了系统性RNAi缺陷 (SID) 1蛋白与双链RNA (dsRNA) 结合的结构. 这揭示了SID1如何识别和结合dRNA,这对于基于RNA的疗法至关重要.

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Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
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相关实验视频

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

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Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
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Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

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科学领域:

  • 结构生物学 结构生物学
  • 分子生物学分子生物学
  • 生物化学 生化学

背景情况:

  • 系统性RNAi缺陷 (SID) 1家族促进核酸运输,使它们成为RNA治疗的目标.
  • SID1蛋白质对双链RNA (dsRNA) 识别的精确分子机制尚不清楚.

研究的目的:

  • 确定sdRNA识别和SID1蛋白结合的结构基础.
  • 提供关于SID1.1对dSRNA的序列独立结合的见解.

主要方法:

  • 使用冷电子显微镜 (cryo-EM) 解析了Caenorhabditis elegans (c) 单独的SID1和与dsRNA复合的结构.
  • 结构分析的重点是cSID1和dsRNA之间的相互作用,包括离子和键,以及静电电位映射.

主要成果:

  • 冷-EM结构揭示了二维cSID1同时结合两个dSRNA分子.
  • dsRNA在富含β链域 (BRD1和BRD2) 的接口上结合,相互作用涉及基本残留物,酸盐骨干和2'-基团.
  • cSID1上的三个基本区域适合dSRNA的主要槽,使得DNA和RNA之间能够进行序列独立的结合和区分.

结论:

  • 该研究提供了cSID1-dsRNA复合体的高分辨率结构,阐明了dsRNA识别的分子机制.
  • 这些发现对于理解sdRNA吸收中的SID1功能以及开发基于RNA的治疗方法至关重要.