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氨基糖化物耐药性基因 aac(6') - Im (aacA16) 的分布和表达

Christopher J Harmer¹, Messiah J Nelson², Francois Lebreton²

¹School of Life and Environmental Sciences, The University of Sydney, NSW, Australia.

The Journal of antimicrobial chemotherapy

|May 14, 2024

概括

阿米卡辛耐药性基因盒 aac(6') - Im 在各种细菌物种中广泛存在,而不仅仅是Acinetobacter baumannii. 它的存在并不总是与阿米卡辛耐药性相关,强调需要将基因内容与观察到的表型联系起来.

科学领域:

微生物学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

在 aac(6 rifluoromethyl) -Im (aacA16) 基因卡塞特赋予了对阿米卡辛,网尔米辛和托布拉米辛的耐药性.
这种耐药性基因多年来一直在Acinetobacter baumannii全球克隆2中全球传播,通常未被发现.

研究的目的:

为了调查aac(6 rifluoromethyl) -Im基因片段的起源和遗传背景.
为了确定这个重要的耐药性基因的非Acinetobacter来源.

主要方法:

使用MINION长读测序和Unicycler混合组件来分析aac(6 rifluoromethyl) -Im. 的遗传环境.
使用定量逆转录酶PCR来评估基因表达水平.

主要成果:

在泰国 (2016-2019) 的Pseudomonas putida和Enterobacter hormaechei分离物中发现了aac(6 rifluoromethyl) - Im基因.
该基因在染色体和等离子体DNA上发现,具有不同的遗传背景和促进元素.
由于缺少Pc促进物,在一个E. hormaechei分离物中没有表达aac(6 rifluoromethyl) - Im基因.

结论:

aac(6 rifluoromethyl) -Im基因比以前认为的更为广泛,在多种细菌物种和多样化的遗传阵列中发现.
这项研究强调了将抗生素耐药性基因存在与实际表达的耐药性表型相关的关键重要性.

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