子宫内膜的生物传感器能力在一定程度上是由来自概念系衍生细胞外囊泡的改变miRNA载荷介导的
Tiago H C De Bem1,2, Alessandra Bridi2, Haidee Tinning1
1Discovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, Leeds, UK.
概括
子宫内膜是子宫内膜的一部分.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 子宫内膜感知怀孕的能力依赖于发育中的胚胎 (概念体) 的信号.
- 由概念体分泌的细胞外囊泡 (EV) 携带关键的载荷,包括微RNA (miRNA).
- 了解Conceptus EVs如何影响子宫内膜功能是繁殖成功的关键.
研究的目的:
- 调查概念性衍生EV中的miRNA载荷是否介导子宫内膜内生物传感器的能力.
- 为了比较子宫内膜对不同发育潜力的概念的反应 (体内,体外,克隆).
主要方法:
- 不同来源 (体内,体外,克隆) 的Bos taurus胚胎被转移给接受者.
- 从概念条件介质中分离了细胞外囊泡 (EV),并对它们的miRNA载荷进行了测序.
- 进行了子宫内膜RNA测序,以分析基因表达变化.
- 用特定的miRNA模仿剂/抑制剂对子宫内膜上皮细胞进行治疗.
主要成果:
- 在体内和体外/克隆概念之间观察到子宫内膜基因表达的实质性差异.
- 概念衍生型电动汽车在所有组中显示出基本上相似的miRNA货物,只有微小的差异.
- 特定的miRNAs (miR-128,miR-1298) 改变了子宫内膜上皮细胞的蛋白质含量,影响了关键基因.
结论:
- 子宫内膜的生物传感器能力在一定程度上是由其对概念性衍生EVs中不同的miRNA载荷的反应介导的.
- 在怀孕早期,EV介导的miRNA转移在调节子宫内膜基因表达方面发挥着作用.
- 这项研究强调了在植入过程中概念体和母体组织之间的复杂沟通.
更多相关视频
10:37Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
8.3K
07:46Author Spotlight: Advancing Reproductive Immunology with a Protocol for the Quantitative Evaluation of Endometrial Immune Cells
Published on: October 13, 2023
1.2K
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:36Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K