生物催化与现场产品去除改善了p-酸的生产
Alexander Virklund1, Alex Toftgaard Nielsen2, John M Woodley1
1Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs., Lyngby, Denmark.
Chembiochem : a European journal of chemical biology
|May 14, 2024
概括
通过在现场去除产品,提高了生产p-酸的生物催化剂. 这种采用水性双相系统的方法,提高了产量1.9倍,克服了酶抑制的挑战.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 酶催化酶的催化作用
背景情况:
- 自然的p-coumaric酸具有有价值的应用,但生物处理产量不足.
- 生物催化剂具有前景,但由于产品抑制了铁氨酸溶解酶而受到限制.
- 脱离增长和生产是提高生物催化剂效率的关键策略.
研究的目的:
- 重新检查和改进生产p-酸的生物催化工艺.
- 为了解决产品抑制铁氨解酶 (TAL) 的问题.
- 使用水性双相系统 (ATPS) 实施现场产品清除 (ISPR).
主要方法:
- 一个聚合物/盐水两相系统被确定用于p-酸提取.
- 在考虑盐度和聚合物类型的情况下,ATPS针对TAL活性进行了优化.
- 在ISPR的ATPS中使用了固定TAL生物催化剂.
主要成果:
- 选择了一种使用5%酸和12.5%聚乙烯甘醇-兰-甘醇的ATPS.
- 优化的ATPS允许高效提取p-coumaric酸,分配系数高达13.
- 在ATPS中固定的TAL生物催化剂在24小时内达到33.5g/L的p-coumaric酸,增加了1.9倍.
结论:
- 使用优化的水性双相系统在现场去除产品有效地克服了TAL产品的抑制.
- 这种增强的生物催化工艺显著改善了p-酸生产指标.
- 开发的方法为有效和可扩展的p-coumaric酸生产提供了一个可行的策略.
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