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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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Updated: Jun 16, 2026

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes

Published on: July 28, 2016

在T细胞等离子体膜上可视化HIV-1组合,使用单分子定位显微镜.

Rayane Dibsy¹, Kaushik Inamdar¹, Cyril Favard¹

¹CNRS, University of Montpellier, Institut de Recherche en Infectiologie de Montpellier - IRIM, UMR9004, Montpellier, France.

Methods in molecular biology (Clifton, N.J.)

|May 14, 2024

概括

超高分辨率显微镜可实时可视化人体免疫缺陷病毒1型 (HIV-1) 口蛋白组合. 这种先进的技术允许详细研究宿主细胞中单分子水平的病毒复制.

关键词:

艾滋病病毒-1 艾滋病病毒-1 棕树的棕树是什么意思这是一场风暴.单分子光显微镜光学显微镜在TIRF-M中使用.病毒的蛋白质组合组

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Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Published on: October 7, 2010

相关实验视频

Last Updated: Jun 16, 2026

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes

Published on: July 28, 2016

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Published on: October 7, 2010

科学领域:

细胞生物学细胞生物学
病毒学病毒学
显微镜的使用方法

背景情况:

光学光显微镜的进步使纳米级生物过程的可视化成为可能.
超分辨率显微镜和光探针允许研究活细胞中单颗粒或蛋白质水平的病毒复制.

研究的目的:

介绍一项可视化人类免疫缺陷病毒1型 (HIV-1) 在宿主T细胞等离子体膜上的口腔组合的协议.
详细介绍使用超分辨率光显微镜研究病毒蛋白组合的方法.

主要方法:

使用全内部反射光显微镜 (TIRF-M) 结合单分子定位显微镜 (SMLM).
描述TIRF设备,HIV-1的T细胞培养,PALM和STORM的样本准备,采集协议和Gag组装集群分析.

主要成果:

通过TIRF-M和SMLM,可以检测和描述宿主细胞等离子体膜中的病毒蛋白组合.
对HIV-1 Gag组件的单个蛋白质水平分析是可以实现的.

结论:

超分辨率显微镜为研究病毒组装动态提供了强大的工具.
描述的协议有助于在分子水平上详细研究HIV-1复制机制.