在麻疹病毒感染的空气液接口培养不朽的人类气道上皮细胞
Justin W Kaufman1, Brajesh K Singh1, Ni Li2
1Stead Family Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA.
Methods in molecular biology (Clifton, N.J.)
|May 14, 2024
概括
本研究详细介绍了在空气-液体接口 (ALI) 上培养人类呼吸道上皮细胞的方法,以模拟麻疹病毒 (MeV) 感染. 这些ALI模型对于理解MeV从人类宿主中退出至关重要.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 麻疹病毒 (MeV) 在呼吸道上皮细胞中复制,促进其释放到环境中.
- 现有的细胞模型如Caco2和MDCK缺乏特定的气道相关性.
- 人类呼吸道上皮细胞对于研究MeV传播动态至关重要.
研究的目的:
- 描述使用空气-液体接口 (ALI) 技术培养人类呼吸道上皮细胞的方法.
- 建立可靠的模型来研究麻疹病毒 (MeV) 感染和从人类呼吸道上皮质中脱出.
- 为研究其他呼吸道病毒在相关细胞环境中提供一个框架.
主要方法:
- 利用了四种不同的人类呼吸道上皮细胞系:16HBE14o-,Calu-3,H358和NuLi-1.
- 建立了空气液体接口 (ALI) 培养条件,以模仿体内呼吸道环境.
- 开发了通过体电阻 (TER) 评估细胞极性和为显微镜准备样本的协议.
主要成果:
- 在ALI条件下成功培养并保持分极化的人类呼吸道上皮细胞.
- 证实了用于确认上皮完整性 (TER) 和为MeV感染研究准备细胞的方法.
- 介绍了顶部和底侧MeV传递的预期结果,强调了模型的实用性.
结论:
- 在ALI培养的极化人类呼吸道上皮细胞为MeV宿主退出研究提供了相关的模型.
- 这些ALI培养方法可用于研究其他呼吸道病毒.
- 已建立的协议推动了对呼吸道上皮细胞生物学和病毒病原学的研究.
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