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通过激光编辑在冷保护样本中逆转结晶

K Rolle¹, K A Okotrub¹, E A Evmenova¹

¹Institute of Automation and Electrometry SB RAS, Academician Koptyug av. 1, Novosibirsk 630090, Russia.

The Journal of chemical physics

|May 14, 2024

概括

这项研究引入了一种新的激光编辑技术,用于修复冷藏过程中冰晶损伤. 通过局部化和再固化晶体,它可以防止进一步的生长,并保持样本的完整性.

科学领域:

低温生物学低温生物学
基于激光的技术.
结晶化结晶化结晶化

背景情况:

结晶是冷保存样品的主要威胁.
目前的冷生物技术专注于冷却/加热,忽视了储存中的维修.
小冰核可以在冷藏期间成长为破坏性晶体.

研究的目的:

提出和评估一种激光编辑技术,用于在冷藏期间修复冰晶.
用聚焦的激光脉冲来研究冰晶的无形化.
评估激光编辑对二甲基硫氧化物三水合物晶体的有效性.

主要方法:

聚焦纳秒激光脉冲用于局部加热和再固化的应用.
拉曼共聚焦映射分析晶体顺序失活.
在激光处理后的加热过程中观察晶体生长.

主要成果:

在冷保护溶液中证明了冰晶的成功无形化.
使用拉曼光谱学识别了二甲基硫氧化三水合物晶体.
在加热过程中显示了激光编辑晶体的增长延迟.

结论:

激光编辑是一种可行的策略,用于修复冷样本中的冰晶损伤.
这种技术通过诱导无形化,有效地阻止了晶体的生长.
这种方法为增强冷保存协议提供了一种新方法.

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