rhesus 的天体细胞细胞系的发展,支持感染一组病毒
Stefanie Reiter1, Ting Sun2, Sabine Gärtner1
1German Primate Center-Leibniz Institute for Primate Research, Infection Biology Unit, Göttingen, Germany.
PloS one
|May 14, 2024
概括
研究人员开发了新的 rhesus 大脑细胞系,这对推动生物医学研究至关重要. 这些对干扰素敏感的细胞支持病毒感染研究,有助于改进非人类灵长类动物模型.
科学领域:
- 神经科学是一个神经科学.
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 非人类灵长类动物 (NHP) 模型对于生物医学研究至关重要,但可用的细胞系稀缺性阻碍了进展.
- 开发新的NHP细胞系对于改进和取代现有的动物模型至关重要.
研究的目的:
- 从 rhesus macaque (Macaca mulatta) 大脑中建立新的细胞系.
- 描述这些细胞系的特征,以了解它们对天体细胞生物学的相关性以及它们在病毒学研究中的有用性.
主要方法:
- 使用c-Fos.的lentiviral转导生成细胞系.
- 转录组分析,免疫阻塞和免疫光显微镜用于细胞特征.
- 评估细胞系对病毒进入的敏感性和对病毒复制的宽容性.
主要成果:
- 已建立的大脑衍生细胞系与星球细胞密切相关,通过状纤维酸蛋白 (GFAP) 表达得到证实.
- 通过定量实时PCR (qRT-PCR) 证明完整的干扰素 (IFN) 系统途径.
- 证实对囊泡性口腔炎病毒 (VSV),淋巴细胞性脑膜炎病毒 (LCMV) 和甲型流感病毒 (IAV) 伪型的敏感性,以及对寨卡病毒 (ZIKV) 和帕皮因疹病毒2 (PaHV2) 的宽容性.
结论:
- 开发了新的,对干扰素敏感的 rhesus macaque 大脑细胞系.
- 这些细胞系对多个病毒糖蛋白具有敏感性,对ZIKV和灵长类简单病毒具有宽容性.
- 新的细胞系为在NHP模型中研究神经向性病毒感染提供了有价值的工具,支持研究改进和替代努力.
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