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相关概念视频

Structure of a Gene01:30

Structure of a Gene

12.5K
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Mechanistic Models: Compartment Models in Individual and Population Analysis01:23

Mechanistic Models: Compartment Models in Individual and Population Analysis

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Mechanistic models are utilized in individual analysis using single-source data, but imperfections arise due to data collection errors, preventing perfect prediction of observed data. The mathematical equation involves known values (Xi), observed concentrations (Ci), measurement errors (εi), model parameters (ϕj), and the related function (ƒi) for i number of values. Different least-squares metrics quantify differences between predicted and observed values. The ordinary least...
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What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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相关实验视频

Updated: Jun 26, 2025

Author Spotlight: Impact of Intergenic Interactions on Disease-Identifying Dark Biomarkers
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Author Spotlight: Impact of Intergenic Interactions on Disease-Identifying Dark Biomarkers

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当将一个简单的电报模型与一个复杂的基因表达模型相匹配时,我们可以学到什么?

Feng Jiao1, Jing Li1, Ting Liu1

  • 1Guangzhou Center for Applied Mathematics, Guangzhou University, Guangzhou, China.

PLoS computational biology
|May 14, 2024
PubMed
概括

简单的随机电报模型准确地捕捉了复杂的基因表达动态,揭示了潜在的调节机制. 该模型的有效参数提供了对基因产品分布和多模式性的洞察.

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Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
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Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 系统生物学 系统生物学
  • 生物物理学的生物物理.

背景情况:

  • 单细胞基因表达分析通常使用随机电报模型来描述mRNA或蛋白质数量的波动.
  • 这种模型通过考虑合成,衰变和基因状态切换 (活性/非活性) 来简化基因动态.
  • 然而,它忽略了复杂的机制,如反,非指数式无活化和多重激活通路.

研究的目的:

  • 为了研究复杂的基因表达模型的动态特性.
  • 评估简单的电报模型能够表示复杂的基因表达分布的能力.
  • 探索电报模型中的有效参数如何揭示潜在的基因调节.

主要方法:

  • 从复杂模型适应稳定状态mRNA或蛋白质数量分布到简单的电报模型.
  • 分析活跃基因状态内的条件分布.
  • 在多个时间点和不同的实验条件下利用单细胞数据.

主要成果:

  • 三参数电报模型准确地捕捉了复杂的基因表达模型的稳定状态和条件分布.
  • 一些有效参数可靠地反映复杂的模型动态,而另一些可能会偏离.
  • 有效的参数可以描述多模式性,并揭示潜在的基因调节机制.
  • 对合作转录调节和外部噪声来说,结果很强大.

结论:

  • 随机电报模型作为复杂的基因表达动态的强大,简化表现.
  • 有效的参数分析提供了一种方法,可以从单细胞数据中推断基因调节机制.
  • 快速放松到稳定状态可以提高参数推断精度,特别是在高外部噪声下.