在HPV阳性宫癌细胞生长中,FOXA1共同激活cirCODC1和ODC1
Rong Jin1, Hongfang Li2, Shoushan Nan3
1Department of Gynaecology and Obstetrics, the Fifth Center Hospital of Tianjin, Tianjin, China.
Systems biology in reproductive medicine
|May 14, 2024
概括
循环RNA ODC1 (circODC1) 通过通过miR-607.7调节ODC1促进HPV阳性宫癌. FOXA1作为circODC1的转录因子,提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 人类乳头瘤病毒 (HPV) 是导致子宫癌的主要原因之一.
- 循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- 在HPV阳性CC中,Hsa_circ_0052602 (circODC1) 表达方式发生变化.
研究的目的:
- 研究circODC1在HPV阳性宫癌中的作用和机制.
- 阐明涉及circODC1,miR-607,ODC1和FOXA1.1的监管网络.
主要方法:
- 定量实时PCR (qRT-PCR) 用于circODC1表达.
- 功能丧失测试评估circODC1在CC细胞生长中的作用.
- 生物信息学和机械分析以确认分子相互作用.
- 染色体免疫沉 (ChIP) 和 luciferase 记者测定对 FOXA1-circODC1 的关联.
主要成果:
- 在HPV阳性CC细胞系中,CircODC1的表达很高,促进了增殖,迁移和入侵.
- CircODC1作为对 miR-607 的竞争性内源RNA (ceRNA) 起作用,上调 ODC1.
- ODC1在HPV阳性CC中表现出致癌作用.
- 与HPV相关的FOXA1被确定为cirCODC1的转录因子.
结论:
- 通过调节miR-607/ODC1轴,CircODC1在HPV阳性CC中发挥着显著的致癌作用.
- 福克斯A1促进circODC1转录,有助于CC的进展.
- CircODC1及其调节途径代表了HPV阳性CC的潜在治疗点.
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