生物相容的等离子体处理液体:一种可持续的方法来消毒致病原体的胃肠道感染
Manorma Negi1, Neha Kaushik2, Prajwal Lamichhane1
1Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, South Korea.
Journal of hazardous materials
|May 14, 2024
概括
冷大气等离子体处理液体对抗抗生素耐药细菌. 这些血处理液体 (PTLs) 诱导活性氧和物种 (RONS),以减少病原体的毒性并促进细菌细胞死亡.
科学领域:
- 生物医学工程 生物医学工程
- 微生物学 微生物学
- 血科学是一门科学课.
背景情况:
- 鼻腔感染是一个重大的挑战,原因是抗生素耐药性和生物膜形成的增加.
- 冷大气等离子体 (CAP) 正在探索生物应用,等离子体处理液体 (PTLs) 提供了更好的便携性和生物相容性.
- 在PTL中,CAP产生的反应性氧和物种 (RONS) 有助于产生协同作用的抗菌作用.
研究的目的:
- 评估血喷射处理的生理溶液 (林格斯乳酸盐,PBS,盐水) 对医院病原体的抗菌疗效.
- 调查PTL抗菌活性背后的机制,包括ROS介导的损伤和对细菌活力和毒性的影响.
- 评估PTLs对细菌基因表达的影响,与病原性和恒温功能相关.
主要方法:
- 使用Ringer的乳酸盐,PBS和用气等离子体喷射处理的生理盐水制备PTL.
- 使用常规培养方法和酸单化物定量PCR (PMAxxTM-qPCR) 评估抗菌活性.
- 分析PTL对细菌活力的影响,诱导活力但不可培养 (VBNC) 状态,以及病毒性相关基因的调制 (例如,S. enterica中的T3SS).
主要成果:
- PTLs对医院和胃肠道病原体表现出显著的抗菌活性,可能是通过ROS介导的脂质过氧化.
- PTLs诱导了可生存但不可培养 (VBNC) 状态,并适度减少了可培养的细菌数量.
- 通过控制S. enterica中与T3SS相关的效应基因,导致PTL暴露导致了致病性功能障碍,表明病毒性调节.
结论:
- 用等离子体处理的液体在诱导ROS介导的细菌损伤方面是有效的.
- PTLs可以控制腹致细菌的毒性,并调节细菌的恒温基因.
- 这项研究强调了PTLs作为抗生素耐药性医院感染的新策略的潜力.
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