氨营养改变了细胞的分布,影响了氨诱导的增长抑制
Agata Wdowiak1, Katsiaryna Kryzheuskaya1, Anna Podgórska1
1Department of Plant Bioenergetics, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
Journal of plant physiology
|May 14, 2024
概括
氨 (NH4+) 营养破坏了植物的 (Ca2+) 稳态,影响了生长. 过度表达CAX1基因通过保持Ca2+平衡和细胞壁完整性,提高了植物对NH4+压力的耐受性.
科学领域:
- 植物生理学 植物生理学
- 营养素管理 营养素管理
- 分子生物学分子生物学
背景情况:
- 均衡的营养供应对于植物的最佳发育至关重要.
- 氨 (NH4+) 和 (NO3-) 是植物的主要源,具有明显的生理影响.
- (Ca2+) 稳态对于植物信号传递,细胞壁结构和整体生长至关重要.
研究的目的:
- 为了研究氨 (NH4+) 营养和 (Ca2+) 稳态在Arabidopsis thaliana之间的关系.
- 阐明NH4+影响植物叶子中Ca2+分布和运输的机制.
- 评估调节Ca2+运输的潜力,以提高植物对NH4+压力的耐受性.
主要方法:
- 用NH4+或NO3-作为唯一源种植的野生类型和突变的阿拉比多普西斯的比较分析.
- 测量Ca2+含量和评估Ca2+在叶子组织中的运输机制.
- 在不同的气条件下对Ca2+载体的基因表达分析.
- 评估CAX1基因过度表达对植物生长和机械特性在NH4+压力下的影响.
主要成果:
- NH4+营养显著扰乱了Arabidopsis叶中的Ca2+稳态,可能是由于透气减少和根到芽的运输受损.
- NH4+压力增加了Ca2+排泄基因在叶子细胞中的表达.
- 过度表达的Ca2+/H+抗载体1 (CAX1) 基因减轻了NH4+压力的负面影响,改善了植物生长和改变了无形成性Ca2+水平.
- 在NH4+压力下,野生类型和oeCAX1植物之间的叶子机械性质 (刚性,强度,性,伸展性) 的不同反应突显了细胞壁代谢的作用.
结论:
- 氨 (NH4+) 作为唯一的源,对植物的Ca2+稳态和生长产生负面影响.
- 减少透气和改变Ca2+运输/外流是NH4+诱导压力的关键机制.
- 调节Ca2+运输,特别是通过CAX1,为增强植物对NH4+毒性和相关生长抑制的耐受性提供了一个有希望的策略.
相关概念视频
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
2.2K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.2K
Synthesis and Functions of Calcitonin
1.8K
Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
1.8K


