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核素93通过Wnt/β-catenin信号调节膀癌中的癌细胞生长和干细胞

Zhe Wang¹, Jing Zhang², Lina Luo¹

¹Urology Department, The First Affiliated Hospital of Hebei North University, No. 12, Changqing Road, Zhangjiakou, 050051, Hebei Province, China.

Molecular biotechnology

|May 14, 2024

概括

核素93 (Nup93) 在膀癌 (BLCA) 中表达高,与预后不佳有关. 向Nup93通过调节Wnt/β-catenin通路来抑制BLCA细胞生长和干细胞.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

膀癌 (BLCA) 是一个重大的临床挑战,治疗选择有限.
核素93 (Nup93) 在BLCA病变发生过程中的作用尚不清楚.
对于BLCA来说,有必要确定新的预后标志物和治疗点.

研究的目的:

研究Nup93在膀癌中的表达和功能.
为了确定Nup93在BLCA患者的预后意义.
阐明Nup93在BLCA中的作用背后的分子机制,特别是它与Wnt/β-catenin通路的联系.

主要方法:

在癌症基因组图谱 (TCGA) 数据集和患者样本中的Nup93表达的分析.
在体外研究中,Nup93在BLCA细胞系中过度表达和敲击,以评估增殖,入侵和干性.
使用分子试验和通路激活剂,研究Wnt/β-catenin信号通路活性.
使用异种移植小鼠模型进行体内验证.

主要成果:

发现Nup93在BLCA组织和细胞系中表达高.
较高的Nup93表达与较差的患者生存结果相关.
努普93敲击抑制了BLCA细胞的增殖,入侵,干细胞和Wnt/β-catenin信号传递.
Nup93的过度表达增强了这些恶性表型.
在体内,Nup93 knockdown显著抑制了瘤的形成.

结论:

Nup93在调节膀癌细胞生长,干细胞和侵袭方面发挥着至关重要的作用.
Nup93通过调节Wnt/β-catenin信号通路来发挥其作用.
Nup93代表了潜在的预后生物标志物和膀癌的治疗点.

关键词:

膀癌是一种癌症.癌症干细胞是一种癌症干细胞.细胞增殖细胞的增殖核聚氨酸 93 93 核聚氨酸在Wnt/β-catenin路径中.

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