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Updated: Jun 26, 2025

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蛋白质适应差异扫描光学使用形状响应性染料

Taiasean Wu1,2, Joshua C Yu1, Arundhati Suresh1

  • 1Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.

Nature biotechnology
|May 14, 2024
PubMed
概括

一个新的蛋白质适应差异扫描度测量 (paDSF) 平台扩展了蛋白质稳定性分析. 这种方法显著增加了与各种蛋白质的兼容性,使新的生物学见解成为可能.

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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