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相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

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相关实验视频

Updated: Jun 26, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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在亚细胞分辨率的空间多组学通过高通量 in situ 双向测序.

Xiaofeng Wu1,2, Weize Xu1,2, Lulu Deng1,2

  • 1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

Nature biomedical engineering
|May 14, 2024
PubMed
概括

我们开发了多omics在现场配对测序 (MiP-seq) 以同时检测DNA,RNA和蛋白质在亚细胞分辨率. 这项技术提高了细胞功能和疾病机制的发现,降低了成本.

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 空间多态技术对于理解细胞功能和疾病机制至关重要.
  • 现有的现场测序方法在解码能力和成本方面存在局限性.

研究的目的:

  • 开发和证明多omics在现场对对测序 (MiP-seq) 的适用性.
  • 为了使DNA,RNA,蛋白质和生物分子在亚细胞分辨率的同时检测.

主要方法:

  • 开发MiP-seq,一种新的现场测序技术.
  • 整合MiP-seq与体内和拉曼成像.
  • 应用一个顺序稀释策略来解决拥挤的信号.

主要成果:

  • 与其他方法相比,MiP-seq提高了解码能力,并降低了测序和成像成本.
  • MiP-seq有效地检测基因突变,等位基因特异性表达和RNA修饰.
  • 产生了老鼠大脑组织的空间多omics地图,将基因表达与神经元活动和生化指纹相关联.

结论:

  • MiP-seq提供了一种强大的,具有成本效益的方法,用于高通量空间多omics分析.
  • 这项技术有助于分子和功能组织图的多维分析.
  • MiP-seq在细胞分辨率下推进了细胞功能和疾病机制的研究.