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对Riccia fluitans L. (Marchantiophyta) 水生过渡使用纳米孔直接RNA测序的Epitranscriptome洞察力

Mateusz Maździarz¹, Katarzyna Krawczyk¹, Mateusz Kurzyński¹

¹Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn, 10-719, Poland.

BMC plant biology

|May 14, 2024

概括

瑞奇亚流通过改变基因表达和RNA修饰来适应陆地和水域. 这项研究揭示了N6-甲基氨酸 (m6A) 和多A尾巴对环境弹性的变化.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
进化生物学进化生物学

背景情况:

里切 (Riccia fluitans) 是一种两性肝,能够适应陆地和水生环境.
了解这种适应的分子机制对于植物生物学和进化至关重要.

研究的目的:

调查在陆地和水生环境之间的过渡期间,Riccia fluitans的表谱变化.
识别差异表达基因 (DEG) 和RNA修饰,如N6-甲基氨酸 (m6A) 和多氨酸) 尾部动力学.

主要方法:

纳米孔直接RNA测序被用来分析转录组.
进行了差异基因表达分析和m6A位点识别.
分析了多A尾长和多基化信号,以了解mRNA的稳定性和翻译.

主要成果:

确定了45个差异表达基因 (DEGs),其中33个在陆地形式下调,12个在水生形式上调.
与陆地形式 (27 个站点) 相比,发现水中形式 (173 个站点) 的N6-甲基氨酸 (m6A) 位点显著增加.
观测到水生形式的多种A) 尾的全球延长偏差,与增加的mRNA稳定性和翻译效率有关,其中9个转录显示了显著的变化.

结论:

里奇亚使用复杂的转录后调节,包括差异甲基化和多基化,以适应不同的环境.
这些发现增强了我们对植物在分子水平上的适应策略的理解.
里奇亚 (Riccia fluitans) 作为一种有价值的模型,用于研究两植物的适应机制.

关键词:

里奇亚的笛子演奏者环境适应环境适应基因表达基因表达甲基化甲基化甲基化非腺因残留物非腺因残留物聚氨酸消毒法聚胺消毒法转录表达方式表达方式

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Ribosome Profiling

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RNA-seq

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