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生成和应用永生羊胎儿纤维细胞细胞系

Guoyu Du^1,2, Cheng Zhang¹, Xiaoan Cao¹

¹State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.

BMC veterinary research

|May 14, 2024

概括

初级绵羊胎儿纤维细胞 (SFFC) 被永久化,以克服研究ORF病毒 (ORFV) 的局限性. 由此产生的永生细胞 (TSFFCs) 保持正常特征,易受ORFV感染,提供了有价值的研究模型.

科学领域:

兽医病毒学兽医病毒学
细胞生物学细胞生物学

背景情况:

初级绵羊胎儿纤维细胞 (SFFC) 对于ORF病毒 (ORFV) 研究至关重要.
局限性包括高成本,寿命短,以及主要SFFC的复杂培养.

研究的目的:

为ORFV研究开发一个不朽的羊胎儿纤维细胞系 (TSFFCs).
为了克服初级SFFCs的局限性.

主要方法:

使用编码SV40大T抗原的lentiviral载体对初级SFFCs进行不朽化.
在裸体小鼠中评估细胞特征,包括蛋白质表达 (纤维菌素,维丁),SV40大T抗原活性,增殖,细胞灭绝耐药性和瘤性.
评估TSFFCs对活体ORFV疫苗的敏感性.

主要成果:

不朽化的TSFFC保留了原始SFFC的生理特征和生物功能.
TSFFCs表现出增强的增殖,延长寿命和对亡的抵抗力.
TSFFCs对活体ORFV疫苗敏感,并且在体内没有恶性病变.

结论:

永久化的TSFFC为ORFV研究提供了强大而安全的体外模型.
TSFFC 适用于病毒隔离,疫苗开发和药物查方面的研究.

关键词:

申请申请表申请表永生的人生不朽.有关ORFV疫苗菌株的疫苗.SV40 大型T抗原绵羊的胎儿纤维细胞.

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