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具有不同单元型的奶牛在S. aureus挑战后显示出不同牛奶细胞数量,牛奶参数和阴道温度的差异,但在E. coli挑战后没有

Katharina Müller-Langhans¹, Lisa Oberberger², Yury Zablotski¹

¹Clinic for Ruminants With Ambulatory Clinic and Herd Health Services, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstrasse 16, Oberschleissheim, 85764, Germany.

BMC veterinary research

|May 14, 2024

概括

乳牛中对乳腺炎耐药性的遗传选择显示了对黄金杆菌挑战的反应的差异. 不同的乳细胞计数 (DMCC) 和阴道温度 (VT) 在有利的 (Q) 和不利的 (q) 类型牛之间有所不同,但在大肠杆菌挑战后没有.

科学领域:

动物科学动物科学
遗传学遗传学是一个
兽医医学兽医医学兽医医学

背景情况:

乳牛的乳腺炎导致重大经济损失,并影响动物福利.
基因选择旨在提高对乳腺炎的抵抗力,乳细胞流细胞计为早期诊断提供了潜在的可能性.
一个标准化的体内模型被用来调查基于特定染色体单元型的乳腺炎的差异敏感性.

研究的目的:

为了比较具有有利的 (Q) 和不利的 (q) Bos taurus染色体18单元型的奶牛之间的差异乳细胞计数 (DMCC),牛奶参数 (脂肪,蛋白质,乳糖,pH) 和阴道温度 (VT).
评估这些参数作为差异性乳腺炎易感性的早期指标的潜力.
评估体内感染模型在研究乳腺炎耐药性的有效性.

主要方法:

利用一个标准化的体内感染模型,对36只半兄弟姐妹的奶牛进行了选择,这些奶牛的父性Bos taurus染色体有18种分歧的哈普洛类型 (Q与q).
牛被感染大肠杆菌 (24小时) 或金黄色葡萄球菌 (96小时),每隔12小时分析样本.
阴道温度 (VT) 被连续记录下来,牛奶样本被分析为DMCC,体细胞计数 (SCC),脂肪,蛋白质,乳糖和pH.

主要成果:

在S. aureus挑战之后,q奶牛显示出较高的PMN水平 (DMCC),SCC和更多的死/活细胞,与Q奶牛相比.
蛋白质和脂肪的百分比在特定时间点在S. aureus挑战后的Q和q奶牛之间有所不同.
阴道温度模式有所变化,最初在q个被S. aureus挑战的奶牛中更高,然后逆转;大肠杆菌的挑战导致S. aureus挑战之前更高的静脉热量.

结论:

活体模型证实了Q和q母牛对金黄色杆菌的明显反应,这种反应在DMCC,牛奶成分和VT中明显.
在E. coli挑战后,没有观察到Q和q奶牛之间的显著差异.
与传统体细胞计数 (SCC) 监测相比,DMCC分析没有提供细化的病原体反应表型.

关键词:

美国大肠杆菌 (E. coli).黄金色的S. 黄金色的贝叶斯模型是贝叶斯模型.牛群是牛群,牛群就是牛群.乳腺内感染模型乳腺炎是一种乳腺炎.牛奶的参数牛奶的参数阴道温度阴道温度

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