在临床隔离的黄金葡萄球菌中检测生物膜生产和抗生素敏感性模式
Sushant Pokhrel1, Namrata Sharma2, Suraj Aryal3
1Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences, Kathmandu, Nepal.
Journal of pathogens
|May 15, 2024
概括
甲素耐药黄金葡萄球菌 (MRSA) 呈现出比甲素敏感黄金葡萄球菌 (MSSA) 更大的生物膜产量. 组织培养板 (TCP) 方法是一种可靠的方法,用于在临床环境中检测金黄色葡萄球菌生物膜的形成.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 医疗器械 医疗器械
背景情况:
- 黄金葡萄球菌 (S. aureus) 的抗生素耐药性和生物膜形成是严重的医疗器械相关感染的主要原因.
- 生物膜中的细菌显著增加了抗生素耐药性,导致慢性感染.
- 有效地检测出S. aureus生物膜的产生对于感染管理和治疗至关重要.
研究的目的:
- 为了比较管和组织培养方法在S. aureus.中检测生物膜产生的有效性.
- 评估甲素抵抗性黄金色杆菌 (MRSA) 和甲素敏感性黄金色杆菌 (MSSA) 隔离物中的抗生素敏感性.
- 评估MRSA和MSSA生物膜形成的能力.
主要方法:
- 用殖民地形态学,格拉姆染色和生物化学测试来识别金黄色菌株.
- 使用Kirby-Bauer光盘扩散方法进行了抗菌素敏感性测试.
- 在MRSA查中,使用了塞福西盘,并通过D-test测试诱导性耐药性. 用两种表型方法评估生物膜形成:TCP和TM.
主要成果:
- 在103个S. aureus分离物中,54个 (52.42%) 是MRSA,49个 (47.57%) 是MSSA.
- 在23/54 (42.59%) 的MRSA分离物中观察到可诱导的MLSB表型.
- 使用TCP方法,26个 (48.1%) 的MRSA分离物是强大的生物膜生产者,而只有6个 (12.2%) 的MSSA分离物.
结论:
- 与MSSA相比,MRSA表现出明显更强的生物膜产量.
- 组织培养板 (TCP) 方法是一种推和可靠的技术,用于检测S. aureus中的生物膜形成.
- 管道方法 (TM) 可以作为常规临床实验室中生物膜生产的有用选工具.
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