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Updated: Jun 26, 2025

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
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Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

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调节冰厚,使用黑猩猩进行高质量的冷EM数据收集

Kelly L McGuire1, Brian D Cook1, Sarah M Narehood1

  • 1Department of Chemistry and Biochemistry, University of California, San Diego, California, US.

bioRxiv : the preprint server for biology
|May 15, 2024
PubMed
概括

高质量的网格准备对于高分辨率单粒子冷电子显微镜 (cryoEM) 至关重要. 像SPT Labtech驼这样的无污点设备可以更好地控制玻璃化,从而提高样品质量.

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction09:25

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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