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Updated: Jun 26, 2025

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

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k无作用 / KI 活细胞中的青素结合蛋白的值确定

Joshua D Shirley, Kelsie M Nauta, Jacob R Gillingham

    bioRxiv : the preprint server for biology
    |May 15, 2024
    PubMed
    概括

    一种新的全细胞测定测量了beta-lactam抗生素对Streptococcus pneumoniae中的青素结合蛋白 (PBPs) 的功效. 这种方法提供了比传统分析更准确的抗生素有效性评估.

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    Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects13:57

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    The Equilibrium Binding Constant and Binding Strength02:18

    The Equilibrium Binding Constant and Binding Strength

    The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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    Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
    Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
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