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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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相关实验视频

Updated: Jun 26, 2025

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

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使用遗传编码的近距离传感器进行传单特异性脂成像.

William M Moore, Roberto J Brea, Caroline Knittel

    bioRxiv : the preprint server for biology
    |May 15, 2024
    PubMed
    概括

    研究人员开发了一种新工具 - - 素激活巧合传感器 (FACES),用于绘制活细胞内的脂质组成和方向图像. 这一突破使得脂质运输和膜不对称性的定量分析成为可能,进步了细胞生物学研究.

    科学领域:

    • 细胞生物学 细胞生物学
    • 生物化学 生物化学
    • 分子成像学分子成像学

    背景情况:

    • 细胞脂质组成在器官和膜叶片中异质.
    • 在高分辨率下测量脂质组成和跨白层方向是具有挑战性的.
    • 现有的工具缺乏用于定量亚细胞脂质分析的特异性和分辨率.

    研究的目的:

    • 引入素激活巧合传感 (FACES),一种用于活细胞中的定量脂质成像的新型化学遗传工具.
    • 调查脂质转移蛋白 (LTP) 在ER和线粒体之间贩运酸胆中的作用.
    • 揭示跨戈尔吉网络 (TGN) 的膜不对称性,并识别有贡献的蛋白质.

    主要方法:

    • 开发FACES,将生物对等化学与遗传编码的素激活蛋白 (FAPs) 结合起来.
    • 应用FACES用于亚细胞脂质池的定量成像及其跨白层方向.
    • 使用含有跨膜域的FAP来评估各种脂类的膜不对称性.

    主要成果:

    • 脸书成功成像了细胞下脂质池及其在活细胞中的定位.
    • 确定了特定LTP在ER和线粒体之间酸丁胆运输中的作用.
    • 揭示了多种脂类TGN的膜不对称性,由细胞质LTP和跨膜飞酶驱动.

    更多相关视频

    Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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    相关实验视频

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    Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

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    Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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    Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

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    Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
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    Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

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  • 证明了FACES用于检测其他分子类的概括性,例如线粒体N-乙赫索胺.

    • 结论:

    • FACES提供了一个强大的,可通用的平台,用于活细胞中的定量,空间定义的分子成像.
    • 该工具阐明了在器官膜上产生和维持脂质不对称的机制.
    • 这项工作推动了我们对细胞膜中的脂质动态和运输的理解.