通过酵母拼接因子Fyv6控制3'拼接地点的选择
Katherine A Senn1, Karli A Lipinski2, Natalie J Zeps1
1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706 USA.
bioRxiv : the preprint server for biology
|May 15, 2024
概括
酵母中Fyv6蛋白的损失会破坏mRNA前拼接,导致广泛使用替代的3'拼接部位. 结构和遗传数据显示Fyv6
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 基因规则 基因规则
背景情况:
- 预mRNA拼接涉及两个催化步骤:5'拼接部位 (SS) 裂变和外链结合.
- 特定的蛋白质因子调节这些步骤,被归类为第一或第二步因子.
- Fyv6 (FAM192A) 之前被确定为酵母中的第二阶段因子,但其更广泛的影响尚不清楚.
研究的目的:
- 为了研究Fyv6对前mRNA拼接的全基因组影响.
- 阐明Fyv6调节拼接的分子机制.
- 为了确定Fyv6功能在spliceosome中的结构基础.
主要方法:
- RNA测序 (RNA-seq) 用于分析 Fyv6 缺乏酵母的转录组范围的拼接变化.
- 高分辨率冷电子显微镜 (cryo-EM) 用于确定结合体结构.
- 基因查以确定Fyv6删除的抑制突变.
主要成果:
- 对Fyv6的损失导致在转录组中激活非共识,分支点 (BP) 邻近3'SS的非共识.
- 化EM结构显示Fyv6是唯一与Prp22 ATPase相互作用的第二步因子.
- Fyv6结合与第一步因子Yju2相互排斥,这表明了监管交换机制.
结论:
- Fyv6在促进共识的使用中发挥着关键作用,BP远距离3' SS在拼接过程中.
- 提出了一个模型,其中Yju2/Fyv6交换促进了高效的外链.
- 了解Fyv6的功能为选择和调节结合部位提供了洞察力.
相关概念视频
01:36Pre-mRNA Processing: RNA Splicing
5.2K
5.2K
01:32RNA Splicing
56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
02:18Alternative RNA Splicing
21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
9.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.3K
02:41Chromatin Structure and RNA Splicing
2.7K
2.7K
02:01pre-mRNA Processing
52.9K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.9K