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对珊瑚的系统学和DNA条形码进行扫描基因组

Andrea M Quattrini¹, Luke J McCartin², Erin E Easton³

¹Department of Invertebrate Zoology, National Museum of Natural History Smithsonian Institution Washington DC USA.

Ecology and evolution

|May 15, 2024

概括

基因组脱皮有效地从珊瑚中提取数千种超保存元素 (UCE) 和外子,解决了家族遗传关系. 这种低成本的方法成功地从历史标本中获取数据,帮助进行分类学修订.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
进化生物学进化生物学

背景情况:

遗传学分辨率对于理解海洋生态系统工程师如珊瑚至关重要.
基因组扫描 (低覆盖基因组测序) 提供了一种有前途的方法,用于提取众多基因组学信息的位置.
该亚属Anthozoa需要改进的遗传学见解.

研究的目的:

评估基因组剥皮对于从珊瑚中提取核位点的实用性.
使用基因组扫描数据重建Anthozoa内部的遗传学关系.
评估将这种方法应用于历史和博物馆标本的可行性.

主要方法:

基因组皮测试是在242个珊瑚标本 (18862022) 上进行的.
使用现有的目标捕获诱集,通过生物信息学提取了超保存元素 (UCE) 和外子.
数据与以前的目标捕获研究相结合;线粒体基因组和核rRNA基因也被恢复.

主要成果:

每个样本平均提取了1837个UCE/外显子 (八角质) 和1379个 (六角质).
植物遗传关系在属类中得到了很好的解决.
从历史标本中成功获取数据,其中最古老的标本来自1886年,平均值为1422个位置.

结论:

基因组脱皮是一种可行的,低成本的方法,用于在Anthozoa.phylogenetic研究.
这种方法可以恢复各种遗传标记,包括UCE,外因子,线粒基因组和rRNA基因.
该方法支持分类学修订和进化历史重建,即使使用历史样本.

关键词:

类动物 (Anthozoa) 是一种动物.在UCE中,UCE是UCE.我们的eDNADNA 博物馆学博物馆学博物馆学目标捕获 - 捕获目标

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