碳氧血红蛋白在不同温度下储存期间的稳定性
Nadine B Wachsmuth1,2, Bassem Bajaa1, Christian Wachsmuth1
1Division of Sports Medicine/Sports Physiology, University of Bayreuth, Bayreuth, Germany.
Drug testing and analysis
|May 15, 2024
概括
碳氧血球蛋白 (COHb%) 度在血液样本中保持稳定,在 -70°C或室温下保存长达12周或6天的血液样本中保持稳定. 这种稳定性确保了准确的血红蛋白质量 (Hbmass) 评估,这对于临床评估至关重要.
科学领域:
- 临床化学 临床化学
- 运动医学 运动医学
- 生物化学 生化学
背景情况:
- 准确的碳氧血红蛋白 (COHb%) 测量对于使用CO-再呼吸方法评估血红蛋白质量 (Hbmass) 是至关重要的.
- 了解血液样本存储期间的COHb%稳定性对于可靠的长期Hb质量分析至关重要.
研究的目的:
- 在血液样本中研究COHb%的稳定性,这些血液样本在不同温度 (+20°C, +4°C和 -70°C) 中长时间存储.
- 为了确定血液储存对血红蛋白质量 (Hbmass) 评估的准确性的影响.
主要方法:
- 来自五名志愿者的血液样本在+20°C和+4°C下保存长达6天后,在-70°C下保存长达12周后,分析了COHb%.
- 血红蛋白质量 (Hbmass) 被测量在13名运动员收集后立即和储存后在+20°C或+4°C3-7天.
主要成果:
- 在+20°C和+4°C下,COHb%在6天内保持稳定,标准偏差最小 (0.07%-0.12%).
- 在70°C下保存12周也显示COHb% (STD0.07%) 没有显著变化.
- 储存3 - 7天后的Hb质量测量显示与立即测量相比,最小差异 (0.8% - 1.1%的典型误差) 很小.
结论:
- 在+20°C和+4°C保存最多6天的血液不会影响COHb%水平.
- 在 -70°C长期储存至多12周,可保持COHb%的完整性.
- COHb%的稳定性确保了可靠的血红蛋白质量 (Hbmass) 评估,即使使用存储的样本.
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