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ZmABF4-ZmVIL2/ZmFIP37模块可以提高玉米苗的干旱耐受性

Dongling Zhang¹, Shixiang Ma¹, Zhixue Liu¹

¹National Key Laboratory of Wheat and Maize Crop Science and Key Laboratory of Regulating and Controlling Crop Growth and Development Ministry of Education, College of Agronomy, Henan Agricultural University, Zhengzhou, Henan, China.

Plant, cell & environment

|May 15, 2024

概括

玉米VIN3样蛋白2 (ZmVIL2) 和FKBP12相互作用蛋白37 (ZmFIP37) 增强了对干旱的抵抗力. ZmABF4转录因子激活ZmVIL2,形成了一条改善植物对干旱压力的耐受性途径.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
无生性压力研究研究

背景情况:

干旱压力显著限制了植物的发展和生产力.
众所周知,PHD基因是应激反应的元素,但它们在无生物应激下对玉米的调节知之甚少.

研究的目的:

研究VIN3样蛋白2 (ZmVIL2) 在玉米干旱耐受性中的作用.
在干旱条件下阐明涉及ZmVIL2的监管网络.

主要方法:

zmvil2突变的识别和特征.
酵母两杂交 (Y2H),双分子光补充 (BiFC) 和共免疫沉 (Co-IP) 试验.
酵母单杂交 (Y1H),电泳运动转移试验 (EMSA) 和双露西法酶记者试验.

主要成果:

zmvil2突变显示干旱敏感性增加,而zmvil2过度表达增强了干旱抵抗力.
ZmVIL2 直接与 ZmFIP37 相互作用,而 zmfip37 淘汰突变体也显示干旱耐受性降低.
发现ZmABF4可以结合ZmVIL2促进体,增强其活性.

结论:

一个新的ZmABF4-ZmVIL2/ZmFIP37路径促进了玉米的干旱耐受性.
这项研究扩大了对玉米PHD基因功能的理解,并为开发耐压品种提供了遗传资源.

关键词:

在 PHD 蛋白质中.干旱造成的压力是干旱.

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