在早期术后肺移植接受者的上下呼吸道微生物组的比较
Chun-Xi Li1, Meng Lv2, Hai-Yue Liu3
1Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Microbiology spectrum
|May 15, 2024
概括
肺移植接受者的上空气道和下空气道微生物群显著不同,采样地点,而不是个体,是微生物变异的主要驱动因素. 这两个地点都含有病原体,反映了移植肺部的健康状况.
科学领域:
- 微生物学 微生物学
- 移植免疫学 移植免疫学
- 基因组学就是基因组学.
背景情况:
- 由于直接连续性,上下呼吸道共享一个微生物群.
- 鼻腔微生物组影响肺微生物组的组成,但肺移植受体 (LTR) 早期术后呼吸道微生物组的了解很少.
- 肺移植的结果比其他固体器官移植更差.
研究的目的:
- 为了比较鼻 (NS) 和支气管洗液 (BALF) 微生物群在早期术后的LTR中.
- 为了确定上空气道和下空气道微生物群之间的差异和相似之处.
- 为了确定采样地点或个体患者是否主要影响微生物组成.
主要方法:
- 16S rRNA基因测序用于分析来自17个早期术后LTR的配对NS和BALF样本.
- 香农多样性和β多样性是为了评估微生物群体结构而计算的.
- 确定了每个区块的核心微生物组.
主要成果:
- 在NS和BALF微生物组之间观察到香农和β多样性的显著差异.
- 确定了不同的核心微生物组:NS样本中含有更高水平的Corynebacterium,Acinetobacter和Pseudomonas,而BALF样本中含有更多的Ralstonia,Stenotrophomonas,Enterococcus和Pedobacter.
- 实验对象内部的不相似性大于实验对象之间的不相似性,这表明采样地点是微生物差异的主要决定因素.
结论:
- 在早期的术后LTR中,上下呼吸道微生物组表现出差异和同质性.
- 在NS和BALF样本中的核心微生物组包括已知的呼吸道病原体,这表明它们在反映移植肺病特征方面的有用性.
- 在LTR中,上空气道和下空气道之间的微生物差异主要是由采样地点驱动的,而不是个体患者.
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