从Viola communis中分离和描述杀虫剂环氧化物
Negin Khatibi1, Yen-Hua Huang1, Conan K Wang1
1Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane QLD 4072, Australia.
这项研究在Viola communis.中发现了20个环状蛋白,其中包括两个新型环状蛋白 (Vcom1和Vcom2). 这些环氧化物显示出作为杀虫剂的潜力,Vcom1比Vcom2.2更强效.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 环类是富含氨酸的类,具有循环的骨干和二硫化物键.
- 它们具有有益的生物物理特性,可用于制药和农业用途.
- 紫罗兰植物家族是环类的主要来源,Viola communis 曾经被推断存在.
研究的目的:
- 调查viola communis.中的环酸的发生,序列和特性.
- 来识别和表征这种植物的新型环氧化物.
- 探索已识别的环旋的潜在杀虫活性.
主要方法:
- 蛋白质组学和转录组学被用来识别循环.
- 质谱法用于的识别.
- 进行了生物测试,以确定细胞毒性和效力.
主要成果:
- 在Viola communis中确定了20个环状蛋白,包括两个新成员:Vcom1 (手子子家族) 和Vcom2 (Möbius子家族).
- Vcom1和Vcom2对Sf9昆虫细胞表现出细胞毒性,而Vcom1更强.
- 在转录组中发现了七个新的环类序列,这表明紫罗兰植物是新型环类的丰富来源.
结论:
- Viola communis含有多种类型的环类,具有潜在的杀虫剂应用.
- 腕环氧化物表现出比Möbius环氧化物更高的杀虫活性.
- 获得了关于环胺加工和生物合成的新见解,建议使用替代的N端加工酶.
更多相关视频
10:35Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea Camellia Sinensis L. Leaves
Published on: June 15, 2019
04:51Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management
Published on: May 26, 2023
相关概念视频
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Voltammetry: Stripping Methods
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Chemical Agents for Microbial Control
Biological Methods for Microbial Control
Microbial Bioremediation of Pesticides
Production of Biopesticides
