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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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数字排序计数允许对目标核酸进行绝对的电量测量.

Yi Liu1, Xu Cui1, Ri Lu1

  • 1Department of Biomedical Engineering and Institute for Health Innovation and Technology, National University of Singapore, Singapore 119077, Singapore.

ACS sensors
|May 15, 2024
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概括

本研究介绍了用于敏感核酸量化的数字排序计数 (DISCO) 平台. 迪斯科提供无标签,绝对量化,没有复杂的光学设置,使得点的护理诊断.

关键词:
数字LAMP是一个数字LAMP.滴滴滴滴滴滴滴滴滴滴滴滴滴滴电气检测检测器电气检测器没有标签的无标签.微流体学 在微流体学方面

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科学领域:

  • 生物技术是生物技术.
  • 分子诊断学 分子诊断
  • 微流体学 微流体学

背景情况:

  • 定量核酸放大测试对于诊断至关重要,但往往需要昂贵的光学设备,限制了它们在实验室外的使用.
  • 目前的核酸量化方法在成本,复杂性和可移植性方面面临着广泛的诊断应用的挑战.

研究的目的:

  • 开发一个微流体平台,用于二进制DNA放大激活滴滴分类和无标签绝对定量核酸.
  • 展示一种新的敏感核酸检测方法,适合于护理场所的环境.

主要方法:

  • 实现一个微流体平台,集成循环介导同热放大 (LAMP) 与按界面张力 (SIFT) 进行分类.
  • 利用LAMP反应引起的pH值变化来对正 (放大) 和负 (非放大) 滴进行分类.
  • 采用芯片上的电极,在排序的滴中数字电气计数目标DNA和RNA.

主要成果:

  • 在微流体平台上成功演示了二进制DNA放大激活滴滴分类.
  • 通过使用数字排序计数 (DISCO) 平台实现了无标签的核酸绝对量化.
  • 验证了目标DNA和RNA的数字电气计数,展示了系统的灵敏度.

结论:

  • 迪斯科平台可以在没有昂贵的光学设置的情况下进行敏感和绝对的核酸量化.
  • 这种基于微流体的方法对于在临床诊断中推进核酸测试具有重大前景.
  • 迪斯科为分子诊断提供了具有成本效益和便携性的解决方案,扩大了超越传统实验室设置的可访问性.