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设计和验证用于通过定量PCR检测和量化环境样本中的抗生素耐药性基因的原料套件

Lizandra Perez-Bou^1,2, Alejandro Gonzalez-Martinez^2,3, Juan J Cabrera⁴

¹Environmental Microbiology Group, Department of Microbiology and Virology, Faculty of Biology, University of Havana, Havana, Cuba.

Microbial ecology

|May 15, 2024

概括

新的qPCR分析有效地监测废水中的抗生素耐药基因 (ARG). 这有助于追踪抗生素耐药细菌 (ARB) 的传播,并保护公共健康.

科学领域:

环境微生物学环境微生物学
分子生物学分子生物学
公共卫生公共卫生

背景情况:

抗生素耐药细菌 (ARB) 对人类健康构成重大威胁.
污水处理厂 (WWTP) 有助于ARB和抗生素耐药基因 (ARG) 的传播.
精确的ARG监测需要使用精心设计的原料进行敏感和强大的qPCR测定.

研究的目的:

开发和验证用于准确量化11个关键ARG的新型原料集.
为在废水环境中监测ARG建立优化的qPCR协议.
提高从WWTP追踪ARG传播的能力.

主要方法:

在基格数据库序列的基础上,针对11个ARG的初始化套件的in silico设计.
用自然和人工废水环境的样本验证原料组和qPCR测定.
测试性能的评估,包括放大效率,线性,可重复性和可重复性.

主要成果:

新的基础套件显示了ARG生物多样性的高覆盖率.
优化的qPCR测定显示出高放大效率 (>90%) 和出色的线性 (R2>0.980).
测试显示出良好的重复性和可重复性,具有广泛的线性动态范围.

结论:

开发的原料组和qPCR方法提供了在WWTP中监测ARG的宝贵工具.
这一进展有助于了解和减轻抗生素耐药性的传播.
改进的ARG监测有助于保护公共卫生和水质.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.抗生素抗生素是一种抗生素.测试优化测试的优化.初级设计设计初级设计初级设计在WWTPs中,WWTPs是WWTPs.qPCRR 是一个很好的方法.

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