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转录因子CgSte12通过影响甲状腺的结构发展来调节致病性Colletotrichum gloeosporioides

Fuhan Li¹, Dongxiao Lu¹, Fanli Meng¹

¹The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, China.

|May 15, 2024

概括

科莱托里 (Collettotrichum gloeosporioides) Ste12 (CgSte12) 对于树中人类病原性至关重要. CgSte12调节了压缩体的发育和结构完整性,影响了真菌的透和疾病的严重程度.

科学领域:

植物病理学植物病理学
分子菌学分子菌学.
菌类遗传学菌类遗传学

背景情况:

科莱托里奇 (Collettotrichum gloeosporioides) 引起花,导致经济和生态的重大损害.
压膜是C. gloeosporioides的重要透结构,粘液的产生和透的形成对宿主入侵至关重要.
在C. gloeosporioides中,CgPmk1是已知的appressorium形成,透和致病性的调节者.

研究的目的:

阐明CgPmk1下游的一种转录因子CgSte12的分子和细胞功能,以及C. gloeosporioides的压缩体发育和病原性.
描述CgSte12在调节压形成和宿主组织透的关键过程中的作用.

主要方法:

对C. gloeosporioidesCgSTE12淘汰突变的生成和表征.
进行比较性转录组分析,以识别由CgSte12调节的基因.
显微镜观察压迫症,包括粘液分泌和透形成.

主要成果:

CgSTE12淘汰突变体表现出正常的细胞生长和形态与正常色的压力.
突变者表现出降低的粘液分泌,受损的压力形成,以及无法形成透钉,导致显著的致病性损失.
转录组分析显示,CgSte12控制了参与appressorium发育的基因,包括与cutinases,NADPH氧化酶和脂质代谢相关的基因.

结论:

在C. gloeosporioides中,CgSte12对于appressorium发育和致病性至关重要.
CgSte12通过调节关键发育和功能基因的表达来调节压力学的结构完整性.
这些发现突出了CgSte12作为管理木花的关键目标.

关键词:

在CgSte12中使用.科莱托里克 (Colletotrichum gloeosporioides) 是一种的植物.压力器是指压力器的压力器.粘结物粘结物粘结物粘结物病原性病原性病原性透挂 (penetration peg) 是一种可以通过透挂来透的方法.

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