相关实验视频

Updated: Aug 11, 2026

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens

Published on: February 20, 2015

对Eimeria tenella ankyrin重复含有蛋白质的分子表征和功能分析

Huilin Guo¹, Qiping Zhao¹, Haixia Wang¹

¹Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Minhang, Shanghai 200241, PR China.

European journal of protistology

|May 15, 2024

概括

在耐药菌株中,Eimeria tenella ankyrin重复含有蛋白质 (EtANK) 的上调,可能有助于寄生虫入侵和耐药性发展. 需要进一步的研究来证实它在肉菌病中的作用.

科学领域:

兽医寄生虫学兽医寄生虫学
分子生物学分子生物学
禽畜科学禽畜科学禽畜科学

背景情况:

由Eimeria物种引起的肉菌病导致全球家禽行业的重大损失.
埃梅里亚 (Eimeria tenella) 是一种流行的埃梅里亚菌种,对严重的菌病负责.
之前的RNA-seq研究表明,在药物敏感和耐药菌株之间,E. tenella ankyrin重复含有蛋白 (EtANK) 的差异表达.

研究的目的:

在E. tenella. 中克隆和分析EtANK的转录和翻译水平.
调查EtANK在E. tenella耐药性和宿主细胞入侵中的作用.

主要方法:

定量实时PCR (qPCR) 用于转录分析.
西方涂抹用于翻译分析.
间接免疫光局部化和体外入侵试验.

主要成果:

与药物敏感 (DS) 菌株相比,耐迪克拉祖里尔 (DZR) 和耐马杜拉米辛 (MRR) 菌株的EtANK表达显著上调.
EtANK蛋白质含量在第二代甲虫 (SM) 中最高,并且局部化到虫 (SZ) 的前部区域和SM内.
一个抗rEtANK抗体抑制了E. tenella对DF-1细胞的入侵.

结论:

埃坦克与E. tenella宿主细胞入侵有关.
EtANK可能对宿主内的寄生虫生长至关重要.
EtANK可能在E. tenella耐药性的发展中发挥作用.

关键词:

安基林是重复含有蛋白质的蛋白质.药物耐药性药物耐药性药物耐药性埃梅里亚天尼拉 (Eimeria tenella) 是一种植物.这是入侵的入侵.

更多相关视频

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Published on: February 14, 2020

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

相关概念视频

Bacterial Protein Maturation

Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...

Amebiasis

Amebiasis

Entamoeba histolytica, a protozoan parasite, is responsible for intestinal and extraintestinal amebiasis. Though a significant proportion of infections remain asymptomatic, approximately 50 million individuals annually are estimated to present with clinical disease, resulting in up to 100,000 deaths globally. The disease burden is disproportionately high in regions with lower socioeconomic status, such as parts of India, Africa, Mexico, and Latin America.Etiology and TransmissionThe infective...