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在晚期产前小鼠中,血前素轴从胎盘转移到垂体起源

Taku James Sairenji¹, Shinnosuke Masuda^1,2, Yuya Higuchi³

¹Department of Integrative Physiology, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan.

Endocrine journal

|May 15, 2024

概括

孕妇的胎盘乳素II (PRL3B1) 水平在怀孕晚期迅速下降,这与之前的研究相矛盾. 这种转变与益乳素激增相吻合,表明激素调节的变化对于维持怀孕至关重要.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
蛋白质组学是指蛋白质组学.

背景情况:

胎盘乳原II (PRL3B1) 是一种类激素,对于维持小鼠怀孕至关重要.
之前的研究报告了产前小鼠中PRL3B1的特定分泌模式.

研究的目的:

在产前小鼠中重新检查PRL3B1的分泌模式.
为了研究在怀孕晚期的激素轴转移和蛋白质组变化.

主要方法:

从妊娠第17天开始,每4小时测量一次母体血PRL3B1和益生菌素 (PRL) 水平.
用RT-qPCR测定胎盘组织中的Prl3b1mRNA水平.
使用数据独立采集质谱法 (DIA-MS) 对胎盘组织进行了枪支蛋白质组分析.

主要成果:

孕妇的血PRL3B1水平从妊娠第17天开始迅速下降,与妊娠第18天的PRL激增相吻合.
孕激素回归先于观察到的前乳素轴转移.
胎盘Prl3b1mRNA水平保持稳定,这表明PRL3B1分泌被抑制.
蛋白质组分析确定了17个PRL家族成员,除了PRL7C1外,大多数成员在胚胎第17天和第18天之间没有显著变化.

结论:

在小鼠中,胎盘中的PRL3B1分泌被抑制在妊娠第18天左右.
孕期晚期发生从胎盘到垂体起源的前乳素轴的转移.
这些发现挑战了之前关于PRL3B1分泌模式的报道.

关键词:

PRL3B1 (益生菌家族3,B子家族,成员1)胎盘中的乳糖原体产前小鼠产前小鼠益生菌 (PRL) 家族中的益生菌 (PRL).蛋白质组学是指蛋白质组学.

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