转录因子AP-2玛通过调节表观遗传修饰来影响猪早期胚胎发育
Daoyu Zhang1, Di Wu2, Sheng Zhang1
1Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, First Hospital, Jilin University, Changchun 130021, China.
Reproductive biomedicine online
|May 15, 2024
概括
转录因子AP-2 (TFAP2C) 对早期猪胚胎发育至关重要. 其缺乏会破坏基因表达,并改变表观遗传修饰,包括基因素和DNA甲基化模式.
科学领域:
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 早期胚胎发育是一个复杂的过程,由精确的基因表达和表观遗传控制来调节.
- 转录因子在编排发育事件中起着至关重要的作用.
- 转录因子AP-2 (TFAP2C) 在猪早期胚胎发生过程中的特定作用在很大程度上仍未被描述.
研究的目的:
- 阐明转录因子AP-2 (TFAP2C) 在猪胚胎早期发育中的作用和作用机制.
- 调查TFAP2C缺乏对基因表达和表观遗传修饰在早期胚胎发育期间的影响.
主要方法:
- 在体外受精 (IVF) 之前,在猪卵细胞中使用siRNA击败了TFAP2C.
- 用RNA测序和定量PCR进行基因表达分析.
- 使用免疫光染色,染色蛋白免疫沉和双光酶记者测定来评估表观遗传修饰和TFAP2C结合.
主要成果:
- TFAP2C缺乏导致发育障碍,显著下调 (1208个基因) 和上调 (792个基因) 的基因表达.
- 淘汰胚胎表现出改变的表观遗传情景,包括增加KDM5B和SETD2的表达,减少H3K4me3/H3K4me2,增加H3K36me3和DNA甲基化.
- TFAP2C直接与SETD2促进体结合,影响其活性,并有助于表观遗传失调.
结论:
- TFAP2C对于正常的早期猪胚胎发育至关重要.
- TFAP2C通过调节组素修饰和DNA甲基化来调节胚胎发育.
- 破坏TFAP2C功能会导致显著的表观遗传变化,影响发育轨迹.
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