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Updated: Jun 26, 2025

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

在酸性pH下持续的细菌N₂O降低

Guang He^1,2, Gao Chen^2,3, Yongchao Xie^2,4

¹Department of Biosystems Engineering and Soil Science, The University of Tennessee, Knoxville, Knoxville, TN, 37996, USA.

Nature communications

|May 15, 2024

概括

微生物的氧化 (N₂O) 减少发生在酸性土壤中. 一种共同培养在pH值为4.5时显示了N₂O的消耗,这是由物种间的营养交换驱动的.

科学领域:

环境微生物学环境微生物学
生物地质化学生物地质化学
土壤科学土壤科学

背景情况:

氧化 (N₂O) 是一个强大的温室气体,增加了农业的排放.
微生物N₂O减少到N₂是一个关键的去除途径,但在酸性环境中通常被认为是可以忽略不计的.
酸性土壤含有nosZ基因,这表明N₂O减少的可能性.

研究的目的:

在酸性条件下研究微生物N₂O减少.
从酸性热带森林土壤中特征化一种微生物共同培养,能够减少N₂O.
阐明在酸性环境中使N₂O消耗成为可能的机制和相互作用.

主要方法:

从酸性热带森林土壤中分离和培养微生物共同种植.
在低pH下N₂O还原活性的生理特征.
综合的奥米克 (基因组学,转录组学,蛋白质组学) 和代谢分析.
酸盐发酵和变性N₂O还原试验.

主要成果:

一种共同种植,包括Serratia sp. 和Desulfosporosinus sp.,在pH值4.5.5时有效降低了N₂O.
共同种植表现出双模生长,其中包括Serratia sp. 发酵的酸盐和Desulfosporosinus sp. 这种植物. 进行性N₂O减少.
确定了物种间的相互作用,其中包括Serratia sp. 为Desulfosporosinus sp.提供必需的氨基酸.
与生长相关的N₂O减少在4.5至6的pH范围内观察到.

结论:

在酸性土壤中,微生物N₂O的减少是可行的,并且是显著的.
同文化动态和物种间的营养交换对于酸性环境中N₂O消费至关重要.
这些发现凸显了以前低估的微生物N₂O在酸性生态系统中的缓解潜力.

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