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Updated: Jun 26, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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人类延长器复合体的冷电磁结构在工作中

Nour-El-Hana Abbassi1,2, Marcin Jaciuk1, David Scherf3

  • 1Małopolska Centre of Biotechnology (MCB), Jagiellonian University, Krakow, Poland.

Nature communications
|May 15, 2024
PubMed
概括

延长器综合体 延长器综合体

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Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

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A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen06:42

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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