相关实验视频

Updated: Jun 26, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.4K

内核酶Q作为核酸放大强化的强大的增强剂

Miyako Shiraishi1, Noboru Nabeshima2, Keiichiro Suzuki3

  • 1Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Japan; Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyamacho, Toyonaka, Osaka, Japan.

Analytical biochemistry
|May 16, 2024
PubMed
概括

一种新的核酸放大方法NAQ简化了DNA放大,在标准反应中添加了内核酶Q和dUTP/dITP. 这种技术避免了复杂的原料设计和特定的酶,增强了分子诊断和生物技术.

关键词:
细胞内核酶 Q 的内核酶 Q.异热放大器的同热放大方式尼克·凯斯 (Nickase) 是一个名人.核酸是核酸中的一种.乌拉是一种酸盐.在 dUTPTP 中使用.

更多相关视频

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

964
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

648

相关概念视频

RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.3K