细菌的囊酸盐异样化途径涉及一个赛马酶和d-囊酸盐硫溶酶
Chunxiu Liu1, Kailiang Ma1, Li Jiang1
1New Cornerstone Science Laboratory, School of Pharmaceutical Science and Technology, Faculty of Medicine, Tianjin University, Tianjin, China.
The Journal of biological chemistry
|May 16, 2024
概括
比洛菲拉·瓦斯沃西亚通过一种新的途径代谢l-囊酸盐,其中包括囊酸盐赛马酶 (BwCuyB) 和d-囊酸盐硫酶 (BwCuyA). 这一过程产生硫化 (H2S),与肠道炎症和结肠癌有关.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 比洛菲拉·瓦斯沃西亚 (Bilophila wadsworthia) 是一种肠道病原生物,它会代谢硫酸盐,产生硫酸盐和硫化 (H2S).
- B. wadsworthia 的 H2S 生产与肠道炎症和结肠癌有关.
- 负责在B. wadsworthia中代谢l-cysteate的特定酶,这是l-cysteine的氧化产物,以前是未知的.
研究的目的:
- 为了阐明在Bilophila wadsworthia中l-cysteate异样化的酶途径.
- 描述参与囊酸代谢的酶及其基质特异性.
- 研究这些酶在其他细菌物种中的更广泛分布和潜在作用.
主要方法:
- 酶表征和活性测试.
- 蛋白质结构建模.
- 用特定的电子受体进行细菌生长实验.
- 跨多种细菌物种的基因同类分析.
主要成果:
- 确定了一条两种酶的途径:囊酸盐赛马酶 (BwCuyB) 将l-囊酸盐异构成d-囊酸盐.
- d-酸盐硫酸酶 (BwCuyA) 将d-酸盐切割成酸盐,氨和硫酸盐.
- BwCuyA对d-cystate具有很高的选择性;同类物在细菌中广泛存在.
- B. wadsworthia的生长与囊酸诱导的BwCuyA表达和H2S生产.
结论:
- 在Bilophila wadsworthia中发现了一种用于d-囊酸盐代谢的新途径.
- 这种BwCuyA酶是一种d-cysteate硫酸酶,与以前关于同类的假设相反.
- 这一途径有助于肠道中H2S的产生,这对炎症性疾病有影响.
- 广泛存在的BwCuyA和BwCuyB同类体表明,在各种环境中,它们在细菌囊酸盐降解中起着重要作用.
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